Simultaneous detection system for multiple disease markers in blood using aptamer set

2014 Fiscal Year Final Research Report

• Project/Area Number: 24360338
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: IKEBUKURO kazunori 東京農工大学, 工学(系)研究科(研究院), 教授 (70251494)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アプタマー / 疾病マーカー / 同時検出 / バイオ関連機器 / 診断技術

Outline of Final Research Achievements

We developed the method to simultaneously detect multiple disease markers in blood using a set of aptamers against various disease markers in blood. We immobilized the various markers in blood onto the beads and added a set of aptamers onto that. We then extracted the bound aptamers from the beads and determined those sequences. We can estimate the concentration of the specific disease marker by counting the sequences of its specific aptamer.
We checked our proposed detection system worked but the background signal from the unspecific binding of the aptamers to the beads or other proteins apart from the target protein is so high that it seemed hard to realize the highly sensitive detection at this time. We found the advantage of the use of boronic acid immobilized bead, which does not seem to affect the structure of proteins upon immobilization. That would become important tool for development of the detection system using aptamers.

Free Research Field

進化分子工学