Mechanistic analyses of chromatin structural changes through the structure of TFIID

2014 Fiscal Year Final Research Report

• Project/Area Number: 24370050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: High Energy Accelerator Research Organization
• Principal Investigator: SENDA Toshiya 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 教授 (30272868)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: X線結晶構造解析 / クロマチン / ヌクレオソーム / 転写 / TFIID

Outline of Final Research Achievements

Nucleosomes are the fundamental repeating units of chromatin. To initiate the transcription reaction, histones must be removed from genomic DNA at gene coding regions. Previously, we determined the cocrystal structure of CIA and double bromodomain, and proposed a recruitment and disassembly model (hiMOST model). In this study, we established the methods for large-scale purification of the HAT domain of TAF1, and the conserved domain of TAF7. In order to perform functional analysis of TFIID in chromatin transcription, we also tried to reconstitute the complex of TBP and TFIIB on promoter DNA.

Free Research Field

結晶学・構造生物学