Regulation of the initiation of DNA replication and its higher order regulation

2015 Fiscal Year Final Research Report

• Project/Area Number: 24370075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: Tanaka Seiji 国立遺伝学研究所, 細胞遺伝研究系, 助教 (50263314)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: DNA複製 / DNA複製開始

Outline of Final Research Achievements

In order to understand the detailed mechanism of the initiation of DNA replication in eukaryotic cells, we have developed a novel strategy to isolate mutants in budding yeast and have isolated tight mutants of replication factors. By using these mutants, we have analysed the mode of association of replication factors to the origin of DNA replication on chromosomal DNA and have revealed that the molecular identity and the mode of assembly of ‘pre-initiation complex (pre-IC)’, which was proposed to exist temporally just before the initiation of DNA replication and was never proved that there. The assembly of pre-IC was mutually dependent on factors that constitute the pre-IC. Such mode of regulation might contribute to the biological robustness of the initiation reaction, and finally contribute to the precise regulation of cellular proliferation.

Free Research Field

分子生物学