2014 Fiscal Year Final Research Report
Functional analysis of Rab GTPase-activating proteins that are recruited to autophagosomes
| Project/Area Number |
24370077
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | Rab / Rab不活性化因子 / 膜輸送 / Atg16L1 / オートファジー / 低分子量G蛋白質 / オルガネラ / 蛋白質分解 |
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| Outline of Final Research Achievements |
It has recently been reported that several Rab GTPase-activating proteins (GAPs) localize autophagosomes, but their precise mechanisms of autophagosomal targeting and their functions in autophagy are poorly understood. In this study, we analyzed the LC3 recognition sequence of OATL1, a Rab33B-GAP, and its localization on autopagosomes. We found that OATL1 is specifically localized outside the autophagosomal membrane through interaction with LC3 and that such asymmetric localization enables OATL1 to regulate the autophagosomal maturation without OTAL1 being degraded by autophagy.
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| Free Research Field |
細胞生物学
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