2015 Fiscal Year Final Research Report
Elusidation of cartwheel-independent mechanisms for establishing 9-fold symmetry of the cdentriole structure
| Project/Area Number |
24370079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Hosei University (2015)
The University of Tokyo (2012-2014) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 細胞骨格・運動 / 微小管 / 基底小体 / 繊毛 |
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| Outline of Final Research Achievements |
Centrioles are organelles with a cylindrical shape consisting of nine triplet microtubules. This 9-fold symmetrical structure is highly conserved among centrioles of most eukaryotic organisms. We previously reported that the cartwheel, a subcentriolar structure with 9-fold symmetry, largely contributes to establishment of the 9-fold symmetry of the centriole. In this study, we produced a cartwheel protein that assembles in vitro into cartwheel-like structures with 6-fold symmetry, and expressed it in Chlamydomonas cells. Observations of the centrioles and cartwheels in the cells suggested that a cartwheel-independent mechanism exists in the circular arrangement of the centriolar microtubules, and that a dynamic interaction between the mechanism and the cartwheel precisely determines the number of the triplets in the centriole.
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| Free Research Field |
細胞生物学
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