Study of the roles of Ser/Thr-Pro consensus motifs for Cdk1 in mitotic regulation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24370083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: SAGATA Noriyuki 九州大学, 理学(系)研究科(研究院), 教授 (80142024)
• Co-Investigator(Renkei-kenkyūsha): NAKAJO Nobushige 九州大学, 理学研究院, 講師 (90294876)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞周期 / M期 / Cdk1キナーゼ / リン酸化モチーフ / ツメガエル卵

Outline of Final Research Achievements

In this study, first we identified the consensus sequence of the novel phosphorylation motif (non-S/TP motif) of Cdk1 and clarified the relationship between Cdk1 phosphorylations of the non-S/TP motif and the classical S/TP motif. Furthermore, we showed the binding of multiple M phase kinases to the S/TP motif and also the mode of the bindings. Moreover, we demonstrated the concerted regulation of the M phase by binding of the multiple M phase kinases to the S/TP motif. Thus, our study showed the molecular basis of the mechanism by which Cdk1 functions as the master M phase kinase.

Free Research Field

生物学