2015 Fiscal Year Final Research Report
Functional analysis and its application of transcription factors responsible for fruit ripening using array technique, transgenic and TILLING technologies
| Project/Area Number |
24380023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Okayama University |
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Principal Investigator |
KUBO Yasutaka 岡山大学, その他の研究科, 教授 (80167387)
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| Co-Investigator(Kenkyū-buntansha) |
YANO Kentaro 明治大学, 農学部, 准教授 (00446543)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Ryohei 岡山大学, 大学院環境生命科学研究科, 准教授 (70294444)
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| Research Collaborator |
Yan Rui
Mworia Eric G.
Asiche William O.
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 果実発育・成熟 / エチレン / アレイ解析 / 形質転換体 |
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| Outline of Final Research Achievements |
Understanding of regulatory mechanism in fruit ripening is central hot point in horticultural physiology and critical point for technology development of storage and transportation. Using a tomato DNA macroarray consisting of 11,520 genes, we identified 419 ripening-associated genes (224 upregulated ,195 downregulated) and isolated 8 key transcription factors. GRAS-suppressed RNAi transgenic tomato exhibited down-regulation of PG and PL genes, suggesting That GRAS plays a key role for regulation of fruit ripening, in particular for softening process.
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| Free Research Field |
園芸利用学
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