Elucidation of global network of regulatory mechanism in virulence-related gene expression of phytopathogenic bacteria

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: ICHINOSE YUKI 岡山大学, その他の研究科, 教授 (50213004)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 病原力遺伝子 / 転写因子 / 菌体密度感知機構 / HrpタイプIII分泌システム

Outline of Final Research Achievements

Motility in Pseudomonas syringae requires flagella and type IV pili (T4P). Flagella and T4P are required for production of acyl homoserine lactones (AHL), quorum sensing molecules and cAMP, respectively. Production of AHL and cAMP also requires transcription factors AefR and PsyR and Vfr, respectively. Defect of aefR or psyR resulted in the enhancement of multidrug efflux pump genes mexEF/oprN, whereas defect of vfr resulted in the enhancement of another pump genes mexAB/oprM. Enhancement of both pump genes activated multidrug tolerance. PsyR and Vfr are also involved in the regulation of hrp gene expression.

Free Research Field

植物病理学