Analyses of the roles of disulfide bond formation between the replication proteins of tomato mosaic virus in viral RNA replication

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant pathology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: ISHIKAWA Masayuki 独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, ユニット長 (70192482)
• Co-Investigator(Renkei-kenkyūsha): ISHIBASHI Kazuhiro 独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, 任期付研究員 (20611742)
• Research Collaborator: CHUJO Tetsuya
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ウイルス / RNA / 複製

Outline of Final Research Achievements

The 130K replication protein of a tobamovirus bound to a 5'-terminal ca. 70-nucleotide region of its genomic RNA to form a ribonucleoprotein complex named PMTC. The binding inhibited translation of the RNA and lead it to a replication pathway. The PMTC contained multiple molecules of the replication protein. Interestingly, free (non-membrane-bound) 130K protein did not bind tobamovirus RNA, but the Met-IR fragment that corresponded to the N-terminal two-thirds of the 130K protein did. Thus, 130K protein molecules likely recognize tobamovirus RNA before the completion of their synthesis.

Free Research Field

分子生物学