Study of the avoidance mechanism of reactive oxygen species production based on a change of energy metabolism in plant

2015 Fiscal Year Final Research Report

• Project/Area Number: 24380039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Okayama University
• Principal Investigator: Yamamoto Yoko 岡山大学, 資源植物科学研究所, 教授 (50166831)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Takayuki 岡山大学, 資源植物科学研究所, 助教 (60362985) ; TSUCHIYA Yoshiyuki 岡山大学, 資源植物科学研究所, 技術職員 (60720670)
• Research Collaborator: MUHAMMAD Sameeullah ; KARIYA Koki ; KOMATSU Kazue
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: アルミニウム耐性 / アルミニウム障害 / 液胞プロセシング酵素 / 活性酸素種 / 細胞死 / スクロース輸送体 / スクロースシンターゼ / タバコ培養細胞株

Outline of Final Research Achievements

A major inhibitory factor of crop production in acidic soils is aluminum (Al) ion. Using cultured tobacco cell lines and seedlings of tobacco, we found (1) a novel avoidance mechanism of ROS production in mitochondria by repression of TCA cycle but enhancement of fermentations in cytosol even under normoxia, which is similar to the Warburg effect reported in cancer cells, (2) an increase in Al tolerance by the over-expression of sucrose transporter in the plasma membrane, and (3) the involvement of vacuolar processing enzyme in cell death mechanism under Al stress.

Free Research Field

農学・農芸化学・植物栄養学・土壌学