Regulatory machinery of sulfur assimilation and metabolism in Arabidopsis: Repression of glucosinolates biosynthesis and induction of SULTR2;1 expression under sulfur deficient conditions.

2016 Fiscal Year Final Research Report

• Project/Area Number: 24380040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Kyushu University
• Principal Investigator: Maruyama-Nakashita Akiko 九州大学, (連合)農学研究科(研究院), 准教授 (70342855)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Keywords: 硫黄同化 / 硫黄代謝 / タンパク質間相互作用 / SLIM1 / グルコシノレート / SDI / 転写制御 / ドメイン解析

Outline of Final Research Achievements

Sulfur (S) is one of the essential macro elements and its assimilation and metabolism has a great impact on plant growth. In addition, there are many health-beneficial and stress-relief sulfur-containing compounds in plants and their contents are highly influenced by the S availability. In this study, some regulatory machinery of S assimilation and metabolism has been investigated. We found Sulfur deficiency induced (SDI)1 and 2 function as the repressors of glucosinolates (GSL) biosynthesis. We revealed that SDI1 binds to MYB28, a transcription factor stimulates the expression of GSL synthetic genes, in the nucleus and suppresses the expression of GSL biosynthetic genes. We also determined a sulfur responsive element SURE21 existing in the 3’untranscribed region of sulfate transporter SULTR2;1 and demonstrated its contribution to the induction of sulfate uptake and translocation to shoots. We also found cadmium treatment induces sulfate uptake and its translocation to shoots.

Free Research Field

植物栄養学