Sustainable control of bacterial wilt by jumbo phage technology

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hiroshima University
• Principal Investigator: YAMADA Takashi 広島大学, 先端物質科学研究科, 教授 (40230461)
• Co-Investigator(Kenkyū-buntansha): FUJIE Makoto 広島大学, 大学院先端物質科学研究科, 准教授 (20274110) ; KAWASAKI Takeru 広島大学, 大学院先端物質科学研究科, 助教 (00510299)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: bacteriophage / bacterial wilt / Ralstonia solanacearum / biocontrol / resistance cost / prophage induction / growth inhibition / loss of virulence

Outline of Final Research Achievements

Four major mechanisms have been found to effects host virulence after infection by RSL1: (1) twitching motility and associated virulence were affected in the host cells,(2) among 12 RSL1 genes enhanced during prolonged infection, orf105, orf106, and orf121 worked for growth activation but orf137 and orf209 strongly inhibited the growth, (3) RSL1 induced RSS prophages in the host cells, and (4) phage resistance working in the host was associated with LPS structure and function, so that resistant cells all lost virulence because of LPS deficiency.

Free Research Field

応用微生物学