Elucidation of the molecular mechanisms of biosynthesis and action of novel bacteriocins from lactic acid bacteria and their applications

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kyushu University
• Principal Investigator: ZENDO Takeshi 九州大学, (連合)農学研究科(研究院), 助教 (50380556)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バクテリオシン / 抗菌ペプチド / 乳酸菌 / 細菌 / 応用微生物

Outline of Final Research Achievements

Structures of novel bacteriocins from lactic acid bacteria, such as enterocin NKR-5-3 and enterocin F4-9, were analyzed and determined. Biosynthetic gene clusters of leaderless bacteriocins such as lacticin Q and lacticin Z, and circular bacteriocins such as leucocyclicin Q were analyzed, and the genes responsible for their secretion and self-immunity were identified. Antibacterial action of lacticin Q was found to involve pore formation on cell membrane of target cells as well as accumulation and scavenging ability of hydroxyl radicals in target cells.

Free Research Field

応用微生物学