2014 Fiscal Year Final Research Report
Structural analysis of novel bifidobacterial enzymes degrading human and plant glycans
| Project/Area Number |
24380053
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ASHIDA Hisashi 近畿大学, 生物理工学部, 教授 (40370987)
FUJITA Kiyotaka 鹿児島大学, 農学部, 准教授 (20381189)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 糖質分解酵素 / ビフィズス菌 / 血液型抗原 / β-アラビノオリゴ糖 / 複合糖鎖 |
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| Outline of Final Research Achievements |
We determined the crystal structure of GH127 beta-L-arabinofuranosidase (HypBA1). HypBA1 has a Cys residue at its active center and was the first "cysteine glycosidase". We found four new glycosidases from B. bifidum. Moreover, we revealed the transcription regulation mechanism of the beta-arabinooligosaccharide degradation system in B. longum and investigated characteristics of a HypBA1 homolog.
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| Free Research Field |
農学・農芸化学
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