Elucidation of mobile loop functions in food-related enzymes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380054
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: MIKAMI Bunzo 京都大学, (連合)農学研究科(研究院), 教授 (40135611)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 応用構造生物学 / タンパク質工学 / 酵素工学 / X線結晶構造解析 / 酵素反応機構

Outline of Final Research Achievements

The functions of proteins are usually expressed by conformational changes in loop regions. We have investigated the mobile loops in food-related enzymes that are used in food industry. The mobile loops in the active sites of beta-amylase and alginate lyase that are important for incorporation of substrate and release of product are analyzed by X-ray crystal analyses of their mutant enzymes. The x-ray analysis of the mutants of pro protein-glutaminase (A47Q, A47E) showed that this enzyme can catalyze the reverse reaction in its catalytic pocket. The 24mer peptide derived from pro-region of transglutaminase inhibited the enzyme. We have determined the structure of the enzyme/peptide complex by x-ray analysis.

Free Research Field

農芸化学