Development and application of induced androgenesis without irradiation of eggs

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380100
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: ARAI KATSUTOSHI 北海道大学, 水産科学研究科(研究院), 教授 (00137902)
• Co-Investigator(Kenkyū-buntansha): YAMAHA Etsuro 北海道大学, 北方生物圏フィールド科学センター, 教授 (60191376)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 倍加半数体 / 倍数体 / 配偶子 / クローン / 受精 / 染色体操作 / ドジョウ / ゼブラフィッシュ

Outline of Final Research Achievements

Genetic inactivation of egg nucleus by UV-irradiation is prerequisite to induce androgenesis (all-male inheritance). We found that cold-shock (3°C for 30 min) of loach eggs just after fertilization eliminated egg nucleus together with second polar body release and sperm nucleus (male pronucleus) initiated androgenesis. Cold-shock of eggs fertilized with diploid sperm of tetraploid loach produced viable androgenetic diploid progeny. Heat-shock (42°C for 2 min) 35 min after the cold-shock treatment successfully produced androgenetic doubled haploids with homozygous genotypes in all the loci. In zebrafish, cold-shock (7°C for 30 min) of eggs just after fertilization also induced androgenetic haploids. Subsequent heat-shock of androgenetic haploids at the first cleavage successfully induced doubled haploids, which produced genetically identical spermatozoa. Cloning was succeeded by the second round production of androgenetic doubled haploids.

Free Research Field

水族遺伝育種学