Prion protein binds to aldolase A in intracellular vesicles of bovine intestinal M cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380150
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Tohoku University
• Principal Investigator: ASO Hisashi 東北大学, (連合)農学研究科(研究院), 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): WATANABE Koichi 東北大学, 大学院農学研究科, 助教 (80261494) ; KITAZAWA Haruki 東北大学, 大学院農学研究科, 准教授 (10204885) ; OKADA Natsumi 東北大学, 技術一般職員 (10621584)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: プリオン / アルドラーゼA / M細胞 / 腸管免疫 / マウス腸管上皮細胞 / ウシ腸管上皮細胞 / 侵入機構 / 抗体阻害

Outline of Final Research Achievements

The exact mechanism by which BSE crosses the intestinal barrier is not clear. M cells are able to incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles. This protein was identified as aldolase A, a glycolytic pathway enzyme, using LC-MS/MS analysis. Aldolase A was synthesized and secreted by BIE cells, and increased during M cell differentiation. In the villi of the bovine intestine, aldolase A was detected on the surface of the epithelium and in the mucus droplet of goblet cells. In the FAE of bovine jejunal and ileal Peyer’s patches, aldolase A was localized on the surface and the apical part of the M cells. The binding of rbPrP to aldolase A was clearly detected and inhibited by pre-treatment of anti-aldolase A antibody. Therefore, aldolase A-positive M cells may play a key role in the invasion of BSE into the body.

Free Research Field

解剖生理学