Molecular analysis on neoplastic survival of mast cells in a poor microenvironment

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380168
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: TANAKA Akane 東京農工大学, (連合)農学研究科(研究院), 教授 (80418673)
• Co-Investigator(Kenkyū-buntansha): KISHIMOTO Miori 東京農工大学, 大学院農学研究院, 講師 (50588960) ; MATSUDA Akira 東京農工大学, 大学院農学研究院, 助教 (90613969)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 獣医学 / 腫瘍 / ストレス応答 / 低酸素

Outline of Final Research Achievements

In the current study, I explored the molecular mechanisms which underpins neoplastic proliferation and survival of mast cells in a poor micro environment. Particularly, stress response and intracellular metabolism of malignant mast cells were investigated. Cytokines that induce neovasclarization were up-regulated when mouse bone marrow-derived cultured mast cells were incubated in hypoxic condition. Relative hypoxia, but not hyperoxia, induced degranulation of mast cells in antigen and IgE independent manner. Not only production of angiogenic factors but also glycolysis were promoted in mast cells derived from canine mast cell tumor. In addition, acceleration of endoplasmic reticulum responses was identified in malignant but not normal mast cells. These results suggest that aggravation of above mentioned responses to hypoxia and lack of nutrition might involve in rapid growth of neoplastic mast cells.

Free Research Field

臨床獣医学