Transactivation potencies of peroxisome proliferator-activated receptor alpha by brominated flame retardants

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380179
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Ehime University (2014); Shokei Junior College (2012-2013)
• Principal Investigator: ISHIBASHI HIROSHI 愛媛大学, 農学部, 准教授 (90403857)
• Co-Investigator(Kenkyū-buntansha): ARIZONO Koji 熊本県立大学, 環境共生学部, 教授 (70128148)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 残留性有機汚染物質 / 臭素系難燃剤 / 核内受容体 / ＰＰＡＲα / 環境汚染 / 生態系保全 / リスク評価 / 遺伝子

Outline of Final Research Achievements

The present study assessed the transactivation potencies of the Baikal seal (Pusa sibirica) peroxisome proliferator-activated receptor α (bsPPARα) by brominated flame retardants (BFRs) using an in vitro reporter gene assay. Among seven BFRs treated in a range of 2.4-50 microM concentration, three PBDEs, BDE47, 99 and 153, induced bsPPARα-mediated transcriptional activities in a dose-dependent manner. To compare the bsPPARα transactivation potencies by PBDEs, the present study estimated the perfluorooctanoic acid (PFOA) induction equivalency factors (IEFs). The order of IEFs for the PBDEs was as follows; BDE153 (IEF: 17) > BDE99 (12) > BDE47 (7.4) >> PFOA (1.0) >>> BDE100, BDE154, BDE183 and HBCDs (not activated). This is the first study in which the transactivation potencies of PPARα by PBDEs in aquatic mammals were clarified. Our study demonstrates that the PPARα-mediated response may be a useful biomarker to evaluate potential biological effects of PBDEs in wildlife.

Free Research Field

生態毒性学・環境分子毒性学