Production of novel platform chemicals from lignin by microbial function

2014 Fiscal Year Final Research Report

• Project/Area Number: 24380180
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: NAKAMURA Masaya 独立行政法人森林総合研究所, きのこ・微生物研究領域, 室長 (50353793)
• Co-Investigator(Kenkyū-buntansha): SHIKINAKA Kazuhiro 東京農工大学, 工学系研究科, 助教 (00507189) ; KATAYAMA Yoshihiro 日本大学, 生物資源科学部, 教授 (10214339) ; OTSUKA Yuichiro 森林総合研究所, バイオマス化学研究領域, 主任研究員 (80455261)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: リグニン / プロトカテク酸3,4-ジオキシゲナーゼ / 2-ピロン－4,6-ジカルボン酸 / 3-カルボキシムコノラクトン / バニリン / プロトカテク酸4,5-ジオキシゲナーゼ

Outline of Final Research Achievements

We have produce 2-pyrone-4,6-dicarboxylic acid (PDC) and 3-carboxymuconolactone (3CML) as a platform chemicals with genes coding for vanillin dehydrogenase (lig V), vanillic acid demethylase (van AB), protocatechuic acid 4,5-dioxygenase (lig AB) and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (lig C) from the lignin degrading soil bacterium, Sphingobium sp. SYK-6, and protocatechuic acid 3,4-dioxygenasd (pca HG) from aromatic compound degrader P.putida KT2440, 3-carboxy-cis,cis-muconate lactonizing enzyme gene (pca B) from Neurpspora crassa respectively.
The PDC polyesters bearing the aromatic groups adjacent to the PDC nuclei exhibited high Td5 (5 wt% loss temperature), good enough to utilize them as thermoplastics. The 3-CML was converted into the corresponding acid chloride derivative, 3-CML dichloride and amid derivative with n-hexyl isocyanate respectively for polymerization.

Free Research Field

森林化学