Specific DNA Recognition by Fluctuation of Zinc Finger Protein and Development for Smart Transcription Factor

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Doshisha Women's College of Liberal Arts
• Principal Investigator: SUGIURA Yukio 同志社女子大学, 薬学部, 教授 (40025698)
• Co-Investigator(Kenkyū-buntansha): NEGI Shigeru 同志社女子大学, 薬学部, 特任助教 (50378866)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 生命錯体化学 / 亜鉛フィンガー蛋白質 / 分子認識 / 遺伝子 / バイオテクノロジー

Outline of Final Research Achievements

DNA-binding and -recognition by zinc finger proteins are regulated with flexible interaction between molecule and molecule that change by surrounding environment, but not rigid key and key hole mode. Multi-zinc finger domain is not necessarily module structure of 3 base recognition per 1 finger, each zinc finger is individual and is not equivalent. On the basis of natural zinc finger and mutant zinc finger, relation between characteristic of zinc finger and variation of DNA complex is suggested interestingly. Further, metal-responsible artificial transcription factor was created.

Free Research Field

医歯薬学