2014 Fiscal Year Final Research Report
Development of a novel RNAi medicine based on shRNA expression mini-plasmid
Project/Area Number |
24390027
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Drug development chemistry
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Research Institution | The University of Tokushima |
Principal Investigator |
MINAKAWA Noriaki 徳島大学, ヘルスバイオサイエンス研究部, 教授 (40209820)
|
Co-Investigator(Kenkyū-buntansha) |
ISHIDA Tstsuhiro 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (50325271)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | RNAi創薬 / 4'-チオDNA / PCR / 非天然塩基対 / クリックケミストリー / 免疫応答 |
Outline of Final Research Achievements |
In this study, I aim at a development of novel RNAi medicine based on shRNA expression mini-gene. As a result, I succeeded to prepare the mini-gene via PCR amplification using 2’-deoxy-4’-thionucleoside triphosphates (dSNTPs). In addition, I also developed a practical method to cyclize the resulting mini-gene. The mini-gene showed potent RNAi activity both in vitro and in vivo. In addition, innate immune responses such as type-I INF and inflammatory cytokines were hardly induced by administration of the mini-gene in vivo.
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Free Research Field |
核酸創薬
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