Elucidation of the pathogenic reinforcement action of enterohemorrhagic Escherichia coli produced SubAB

2016 Fiscal Year Final Research Report

• Project/Area Number: 24390104
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Chiba University
• Principal Investigator: Noda Masatoshi 千葉大学, 医学(系)研究科(研究院), 教授 (60164703)
• Co-Investigator(Kenkyū-buntansha): 清水 健 千葉大学, 医学(系)研究科(研究院), 准教授 (70312840) ; 八尋 錦之助 千葉大学, 医学(系)研究科(研究院), 准教授 (80345024) ; 津々木 博康 大阪府立大学, 理学(系)研究科(研究院), 助教 (40586608)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Keywords: 腸管出血性大腸菌 / SubAB / 小胞体ストレス / 一酸化窒素 / 細胞死 / ストレスグラニュール

Outline of Final Research Achievements

Subtilase cytotoxin (SubAB) is mainly produced by locus of enterocyte effacement (LEE) -negative strains of Shiga-toxigenic Escherichia coli (STEC). SubAB cleaves an endoplasmic reticulum (ER) chaperone, BiP/Grp78, leading to induction of ER stress. This stress causes activation of ER stress sensor proteins and induction of apoptosis. Here we showed that 1) SubAB enters the cells via lipid rafts- and an actin-dependent pathways 2)in mouse macrophages, that SubAB inhibited LPS-stimulated NO production through inhibition of NF-kB nuclear translocation and iNOS expression 3)SubAB-induced SG formation is regulated by the PERK/DAP1 signaling pathway, which may be modulated by PKCdelta/PKD1.

Free Research Field

細菌学