Structure and movement of gliding machinery for Mycoplasma gliding

2015 Fiscal Year Final Research Report

• Project/Area Number: 24390107
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka City University
• Principal Investigator: Miyata Makoto 大阪市立大学, 大学院理学研究科, 教授 (50209912)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 細胞運動 / 電子顕微鏡 / 一分子計測 / 病原細菌 / 構造生物学 / 電子線クライオトモグラフィー

Outline of Final Research Achievements

Mycoplalasma pneumonia, epidemic recent years is caused by a bacterium, Mycoplasma pneumoniae. Mycoplasmas glide by quite unique mechanisms, which are essential for infection. In this project, we studied the fasest species, Mycoplasma mobile and the pathogenic Mycoplasma pneumoniae and got the following results, (1) namometer-order three dimensional structure of gliding machinery, (2) identification, detecting activity and outline of structure, for essential proteins, (3) ATPase reaction of gliding machinery, (4) behaviors of “leg” molecule, (5) development of gene manipulation method and localization of gliding proteins.

Free Research Field

バクテリアの細胞生物学