Directing of decdritic cells by Bordetella type III effectors

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390108
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kitasato University
• Principal Investigator: ABE Akio 北里大学, 感染制御科学府, 教授 (50184205)
• Co-Investigator(Kenkyū-buntansha): KUWAE Asaomi 北里大学, 感染制御科学府, 准教授 (60337996)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 百日咳菌 / 気管支敗血症菌 / ボルデテラ属 / III型分泌装置 / エフェクター / BopN / BopC

Outline of Final Research Achievements

Type III secretion systems (T3SSs) are highly conserved in Bordetella pertussis and its related strain, B. bronchiseptica. This machinery delivers effectors into host cells leading to the long term colonization on trachea. Using a CyaA-mediated translocation assay, we demonstrated that BopN has an ability to translocate into the host cells. Furthermore, a pull-down assay revealed that BopN can associate with BopC. Interestingly, BopC-induced host-cell death is facilitated by the BopN localization into host cells.

Free Research Field

医歯薬学