2014 Fiscal Year Final Research Report
Analysis of human innate immunity and acquired immune mechanism during fetal period using human ES cells
| Project/Area Number |
24390272
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
EBIHARA Yasuhiro 東京大学, 医科学研究所, 特任准教授 (40302608)
TSUJI Kohichiro 東京大学, 医科学研究所, 准教授 (50179991)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 胎児医学 / ES細胞 / 自然免疫 |
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| Outline of Final Research Achievements |
We succeeded the production of human mast cells and eosinophils from hematopoietic cells derived human ES cells and established the system that reflect the generation and development of these immune cells in human embryo. It is indicated that they first develop connective tissue-type mast cells that both express chymase and tryptase at primary time of induction and then from multipotential hematopoietic progenitor cells at a comparatively later stage they develop mucosal mast cells. It is also suggested that eosinophils and basophils might be originated from common progenitor cells. We succeeded the production of human neutrophils from hematopoietic cells derived human iPS cells as well. In the analyses, they showed normal and healthy functions, compared with the one derived from iPS cells from patients of congenital neutrophilic disorders.
it is expected to be a useful tool for investigations into the mechanism of the generation of innate immune system and many diseases.
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| Free Research Field |
小児血液
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