Novel neointima-inducing treatment of acellular small-diameter blood vessel for good patency

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390307
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: NAKATANI Takeshi 独立行政法人国立循環器病研究センター, 病院, 部長 (60155752)
• Co-Investigator(Kenkyū-buntansha): YAMAOKA Tetsuji 国立循環器病研究センター, 研究所, 部長 (50243126) ; FUJISATO Toshiya 大阪工業大学, 工学部, 教授 (60270732) ; MAHARA Atsushi 国立循環器病研究センター, 研究所, 研究員 (80416221)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脱細胞組織 / 小口径血管

Outline of Final Research Achievements

The peptide containing collagen binding domain and endothelial cells binding sequence has been used as surface modifier for decellularized blood vessel. In this study, endotherial-cell binding peptide, Arg-Glu-Asp-Val (REDV), was conjugated with collagen-binding sequence (Pro-HydoxyPro-Gly)7 ( (POG)7), and the peptide was　immobilized to the luminal surface of the decellulalized tissue. Endothelial cell binding affinity of the decellularized tissue surface was greatly improved by the peptide modification in vitro. Decellularized rat abdominal aorta with a diameter of ca. 1 mm was prepared by the UHP treatment and peptide modification, and the peptide-modified graft was transplanted into the rat aorta. Very good patency and endotherialization were observed after 1 month transplantation, while the control grafts rapidly occluded. These results suggested that the peptide modification promoted the endotherialization and tissue regeneration on the graft, and lead to the good patency.

Free Research Field

移植医療