Establishment of 4th generation of islet isolation and the clinical application

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390316
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: University of the Ryukyus (2014); Clinical research Center, Chiba-East National Hospital, National Hospital Organization (2013); Okayama University (2012)
• Principal Investigator: NOGUCHI Hirofumi 琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
• Co-Investigator(Kenkyū-buntansha): SAITOH Issei 新潟大学, 医歯学系, 准教授 (90404540)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 膵島移植 / 膵島分離 / JNK抑制剤 / 新規臓器保存液 / 膵島純化 / 連続比重勾配 / 連続密度勾配

Outline of Final Research Achievements

We previously established a new islet isolation protocol(3rd generation) based on the Ricordi method. It includes pancreatic ductal protection, a MK preservation solution, the two-layer method of pancreas preservation, a new islet purification solution (Iodixanol-based solution), supplemental purification, and cold preservation of isolated islets. In this study, we established 4th generation of islet isolation protocol, including HN-1 preservation solution, ductal injection with JNK inhibitor, and controll-density and osmolality solution for islet purification. Establishment of this method enables us to make diabetic patients insulin independent using islets not only from 2 or 3 pancreata of NHBDs, but also from a pancreas from a brain-dead donor.

Free Research Field

膵島移植