Mechanism of malignant transformation progress from bronchioloalveolar carcinoma to invasive adenocarcinoma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390329
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Hiroshima University
• Principal Investigator: OKADA Morihito 広島大学, 原爆放射線医科学研究所, 教授 (70446045)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 肺癌 / 腺癌 / 悪性化

Outline of Final Research Achievements

Early lung adenocarcinoma cell line with constitutive Notch2 overexpression was established. TGF-B and Smad3/4, which are related to promoting epithelial mesenchymal transition, were examined by RT-PCR. Subsequently, Six1, Slug, Snail, and Hey1, down-stream molecules of Notch2 signaling pathway, were also examined.
It is difficult for small lung adenocarcinoma, a target of this study, to be applied for next generation sequencer because of its small amount of RNA. Thus, we performed further investigation of the candidate molecules that have been identified in the previous study. GBP1 was identified as a promising molecule after validation with lung adenocarcinoma cell lines and human lung adenocarcinoma specimen. In addition, wound healing assay and migration assay demonstrated that GBP1 was involved in inducing cancer invasion. We now plan to examine the association between GBP1 expression and Notch or Ras signaling pathway and the prognosis of patients with lung adenocarcinoma.

Free Research Field

胸部外科学