Mechanisms underlying craniofacial development from embryonic stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390411
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: FURUE MIHO 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, 研究リーダー (80257310)
• Co-Investigator(Kenkyū-buntansha): SUGA Mika 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, 特任研究員 (00340448) ; YANAGIHARA Kana 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, プロジェクト研究員 (10571333)
• Research Collaborator: MIMURA Sumiyo 独立行政法人医薬基盤研究所, 難病・疾患資源研究部・ヒト幹細胞応用開発室
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 幹細胞 / 神経堤 / ヒト多能性幹細胞 / ヒト胚性幹細胞 / 無血清培養 / 頭部神経堤 / 発生

Outline of Final Research Achievements

To understand the mechanisms underlying craniofacial development, we promoted cell differentiation of human pluripotent stem cells (hPSCs) into ectoderm, neural epithelium, and anterior neural crest. At first, we have provided evidence that fibroblast growth factor-2 and Activin A synergistically regulated the initiation of hPSC differentiation into neural cell lineages even though hPSCs autonomously differentiate into neural cell lineages. Further, we developed a differentiation protocol for anterior neural crest induction using several growth factors and inhibitors, suggesting the mechanisms underlying craniofacial development.

Free Research Field

幹細胞生物学