Develpment of novel therapy for periodontium regeneration using periodontal iPS cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390426
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: AKAMINE Akifumi 九州大学, 歯学研究科(研究院), 教授 (00117053)
• Co-Investigator(Kenkyū-buntansha): MAEDA Hidefumi 九州大学病院, 歯内治療科, 講師 (10284514)
• Co-Investigator(Renkei-kenkyūsha): WADA Naohisa 九州大学病院, 歯内治療科, 講師 (60380466) ; MONNOUCHI Satoshi 九州大学, 大学院歯学研究院, 助教 (30609558)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 歯根膜組織 / iPS細胞 / 歯周組織再生 / 神経堤細胞

Outline of Final Research Achievements

In this study, we aimed to develop a novel therapy to regenerate defects formed in periodontium using iPS cells. As neural crest cells are thought to be the origin of mesenchymal cells that fabricate the periodontium, we first induced iPS cells derived from human skin fibroblasts to neural crest (NC)-like cells. We then cultured these cells on culture dishes coated by extracellular matrices produced by primary human periodontal ligament (PDL) cells. Consequently we obtained PDL-like cells from NC-like cells, which exhibited the features of PDL cells. In this context, we transplanted such PDL-like cells into the defect of periodontium experimentally created in rat mandibular, and observed the partial regeneration of alveolar bone and PDL tissue after 4 weeks of transplantation histologically. We therefore believe that our results indicated the way to clinically apply iPS cells for periodontal regenerative therapy.

Free Research Field

歯科保存学