The development of new regenerative therapy for periodontal-bone tissue defects by using de-differentiated fat cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24390444
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Kagoshima University
• Principal Investigator: KAZUYUKI Noguchi 鹿児島大学, 医歯学総合研究科, 教授 (90218298)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Toshiaki 鹿児島大学, 大学院・医歯学総合研究科, 助教 (60381183) ; SAKODA Kenji 鹿児島大学, 大学院・医歯学総合研究科, 助教 (70419654) ; YOSHIMOTO Takehiko 鹿児島大学, 大学院・医歯学総合研究科, 客員研究員 (60419653)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脱分化脂肪細胞 / 歯周組織 / 骨 / 再生 / DFAT

Outline of Final Research Achievements

The purpose of this study was established that the molecular biologic base of new cell-transplant therapy for lost of periodontal and bone tissues by using de-differentated fat cells (DFAT). Within the limits of the present study, we demonstrated that the poly lactic-co-glycolic acid/hydroxylapatite (PLGA/ HA) composite is a promising scaffold with an appropriate stiffness and resorption rate, and that its combination with osteo-differentiated rat DFAT may be effective for bone formation in rat calvarial defects model. Furthermore, in vitro study demonstrated that the combination of bone morphogenetic protein (BMP)-9 and FK506 effectively induces osteoblastic differentiation of rDFAT cells.

Free Research Field

歯周病学