Exploration of functional-metal nano particle-producing bacteria from extremophile environments

2015 Fiscal Year Final Research Report

• Project/Area Number: 24405030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Kawamoto Jun 京都大学, 化学研究所, 助教 (90511238)
• Co-Investigator(Kenkyū-buntansha): KURIHARA Tastuo 京都大学, 化学研究所, 教授 (70243087)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 金属ナノ粒子 / 極限環境微生物 / 低温菌 / 菌体外膜小胞

Outline of Final Research Achievements

Metal nanoparticles have significantly different physicochemical characteristics from bulk metal materials, and their catalytic activities have great interests in various fields. In this study, to develop a synthesis method for metal nanoparticles at moderate environments, we attempted to explore metal nanoparticle-producing bacteria and their synthesis mechanism. Pseudomonas sp. H206 isolated from inner Mongolia and Shewanella livingstonensis Ac10 isolated from Antarctic seawater, which produce silver nanoparticles at the extracellular region, secret vesicles surrounded by lipid bilayer, and the size of membrane vesicles are about 100 nm. To elucidate the vesiculation mechanism of S. livingstonensis Ac10, we performed molecular characterization of the membrane vesicles of this strain. Moreover, the production of uniformly sized spherical vesicles might be involved in the synthesis of nano sized metal particle by these microorganisms.

Free Research Field

応用生物化学