2014 Fiscal Year Final Research Report
Establishment of Chernobyl paraffin-embedded tissue Bank and discovering of new oncogene
| Project/Area Number |
24406031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 海外学術 |
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| Research Field |
Endocrinology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Masahiro 長崎大学, 原爆後障害医療研究所, 教授 (50284683)
SAENKO Vladimir 長崎大学, 原爆後障害医療研究所, 准教授 (30343346)
SHIBATA Yoshisada 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (40010954)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 小児甲状腺乳頭癌 / パラフィン組織バンク / 次世代シーケンシング / TBL1XR1/RET |
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| Outline of Final Research Achievements |
During this study we collected from affected after Chernobyl fallout territories of Belarus and Ukraine paraffin-embedded tissues, frozen samples (if available) and blood samples from the most aggressive (young age of exposure and short-latent period) pediatric PTCs (operated from 1990 and until October 1998), which are not included in the Chernobyl Tissue Bank and established the Paraffin Tissue Bank. For all collected PTC samples we made data base of patients includes demographic, clinical and morphological information.
Using RNA-NGS technique we discovered a new RET rearrangement (TBL1XR1/RET) in pediatric PTC from Belarus. In vitro irradiation of human thyroid cells was able to induce TBL1XR1/RET rearrangement. The occurrence of TBL1XR1/RET rearrangement in PTC of sporadic and radiation-related etiology suggests that it may likely be induced by different genotoxic insults, one of which could be ionizing radiation.
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| Free Research Field |
内分泌学,甲状腺学,分子生物学
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