Structural basis for centrifugal regulation of synaptic circuit in the olfactory bulb

2014 Fiscal Year Final Research Report

• Project/Area Number: 24500418
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kawasaki Medical School
• Principal Investigator: KAZUNORI Toida 川崎医科大学, 医学部, 教授 (40253405)
• Co-Investigator(Renkei-kenkyūsha): 金子 武嗣 京都大学, 大学院, 教授 (90177519)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 神経回路 / 嗅球 / セロトニン / 遠心性調節 / シナプス / 単一ニューロン標識 / デジタルトレース / 電子線トモグラフィー

Outline of Final Research Achievements

Olfactory bulb (OB) is an attractive region for analyzing neural circuit in the brain. It has been so far well-examined with special focus on interneurons in the OB. Recent progress in neuronal labeling and biological application by newly developed microscopes enable to reveal long-distance projection neuron from other brain regions to the OB as centrifugal input, which have not been analyzed for overall morphology becoming structural basis for understanding regulatory function to the OB neural circuit. Using combination of transgenic mice, genetic induction by viral vectors, multiple immuno-labeling, correlative laser and electron microscopies, digital tracing of a single neuron, and electron tomography, we have analyzed centrifugal regulation to the OB neural circuit and revealed significance of presence of synapse as regulatory inputs to the OB from other brain regions. We further propose some possibilities in future analyses to be structural basis for neural circuit functions.

Free Research Field

神経解剖学