The role of ROS-generating enzyme on the psychiatric disorders

2014 Fiscal Year Final Research Report

• Project/Area Number: 24500445
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Masakazu Ibi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10336539)
• Co-Investigator(Kenkyū-buntansha): 松本 みさき 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80533926)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 精神疾患 / 活性酸素種

Outline of Final Research Achievements

The involvement of reactive oxygen species (ROS) in psychiatric disorders has been reported. However, the source of ROS has not been identified yet. We investigated the role of NOX1/NADPH oxidase in the depressive-like behavior using mice deficient in Nox1 (NOX1-KO). When wild type mice (WT) were repeatedly subjected to social defeat, the social interactions were decreased (avoidance behavior). Similarly, chronic administration of corticosterone (CORT) reduced sucrose preference (anhedonia). These depressive-like behaviors were significantly suppressed in NOX1-KO. Increased level of NOX1 mRNA was observed in the ventral tegmental area of WT treated with CORT, while the level of NOX1 mRNA was unaltered in the prefrontal cortex (PFC). On the other hand, increased ROS level in PFC of CORT-treated WT was significantly attenuated in NOX1-KO. Concomitantly, decreased levels of BDNF mRNA was significantly blunted in PFC of NOX1-KO.

Free Research Field

薬理学