Relationship between dendritic localization of inhibitory synapses and visual information processing in mammalian retinal ganglion cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24500467
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: KOIZUMI Amane 生理学研究所, 細胞器官研究系, 特任教授 (10296551)
• Co-Investigator(Kenkyū-buntansha): NAKAZAWA Toru 東北大学, 医学(系)研究科(研究院), 教授 (30361075)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 網膜 / 視覚 / 組織培養 / 遺伝子導入 / 樹状突起

Outline of Final Research Achievements

We successfully applied organotypic adult retina culture system with gene-gun to marmoset retina. Especially, we introduced GFP plasmid into marmoset retinal ganglion cells, and found a morphological diversity of retinal ganglion cells beside well-known midget and parasol ganglion cells. In addition, we introduced PSD95-GFP, synaptic maker, plasmid into retinal ganglion cells and analyzed dendritic localization pattern of synapses in different subtypes of retinal ganglion cells. By this method, we found narrow thorny retinal ganglion cells almost exclusively receive synaptic inputs from DB6 bipolar cells, and project into K1 layer of lateral geniculate nucleus.

Free Research Field

神経生理学