2015 Fiscal Year Final Research Report
Identification of specific splicing regulation at neuromuscular junctions
| Project/Area Number |
24500469
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
Masuda Akio 名古屋大学, 医学(系)研究科(研究院), 准教授 (10343203)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 神経筋接合部 / スプライシング / 筋分化 |
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| Outline of Final Research Achievements |
To analyze specific regulation of RNA processing in neuromucular junctions, we harvested RNA from neuromuscular junctions in mouse skeletal muscles with laser microdissection, and performed microarray and RNA-seq analysis. Our analysis revealed that alternative splicing was distinctly regulated at neuromuscular junctions.
We also examined C2C12 cmyotubes stimulated with Agrin/Neuregulin, and found that SR proteins were deeply involved in the specific regulation of mRNA splicing at neuromuscular junctions.
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| Free Research Field |
神経医学、ゲノム科学
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