2014 Fiscal Year Final Research Report
Mechanism of cancer cell apoptosis under low voltage electric pulses
| Project/Area Number |
24500524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Okayama University of Science |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 低電圧パルス / カスパーゼ / 細胞内カルシウム / アポトーシス |
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| Outline of Final Research Achievements |
High-intensity electrochemotherapy has been investigated as a novel anticancer therapy. However, the application of high-intensity electric fields puts great stress on patients. Therefore, cell death induced by a milder electric field would be a favorable alternative to high-voltage electrochemotherapy. In general, mild electric fields appear to induce less cell damage and greater cell survival than high-intensity fields. Research into the cellular effects of exposure to Low voltage electric pulses (LVEPs) is useful for cancer research.
We examined the role of caspases activities and intracellular Ca2+ in the subsequent cellular response. As a result, we showed that a mild electric field could be a favorable alternative to high-voltage electrochemotherapy. Chemotherapy is generally effective in aerobic condition. Therefore, we studied oxygen micro bubble technique to make cancer tissue aerobic condition. We propose new mild electrochemotherapy under aerobic condition.
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| Free Research Field |
医工学
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