An investigation of endocrine factors from cultured skeletal muscle cells and the effect of cell pressurization

2014 Fiscal Year Final Research Report

• Project/Area Number: 24500864
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: IIZUKA Kenji 北海道医療大学, 薬学部, 教授 (10344467)
• Co-Investigator(Kenkyū-buntansha): HIRAFUJI Masahiko 北海道医療大学, 薬学部, 教授 (20142987) ; MACHIDA Takuji 北海道医療大学, 薬学部, 講師 (90433424)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 骨格筋細胞 / ミオカイン

Outline of Final Research Achievements

Human skeletal muscle cells (Huskmcs) were cultured and pressurized to spuriously reproduce an intra-muscular pressure elevation during exercise. Investigations of mRNA expression in pressurized Huskmcs resulted in an increase in several secreted endocrine factors known as myokines, and protein levels in culture medium were also increased compared with non-pressurized cells. An administration of culture medium, collected by pressurized Huskmcs, to human adipocytes resulted in an increase in more than two times of up-regulation in cytokine release into culture medium. Huskmcs may play as a secretory organ during exercise and those released factors may have effect on adipocytes as a myokine.

Free Research Field

総合領域