Integrative genomic and proteomic analyses of low-dose ionizing radiation in EBV infected-B cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24501306
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Carcinogenesis
• Research Institution: Juntendo University
• Principal Investigator: TABE Yoko 順天堂大学, 医学部, 准教授 (70306968)
• Co-Investigator(Kenkyū-buntansha): IWABUCHI Kazuhisa 順天堂大学, 看護学部, 教授 (10184897) ; SASAI Keisuke 順天堂大学, 医学部, 教授 (20225858)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 低線量放射線 / 微小環境 / 前がん細胞 / microRNA / プロテオミクス

Outline of Final Research Achievements

Exposure to low-dose ionizing radiation (LDIR) can alter mammalian cell gene expression. We compared the cellular response of irradiated immortalized, EB virus;infected, B-cells (EBV-B) cultured alone with that of EBV-B cells co-cultured with bone marrow derived stromal cells (MSCs). The miRNAs let7a, miR-15b, miR-16, miR-21 and a lipid metabolic miRNA hub miR-23b were upregulated after LDIR exposure in the mono-cultured EBV-B cells, but were downregulated in EBV-B cells co-irradiated with MSCs. A lipid biosynthesis enzyme GPAM, the common target of these miRNAs, was downregulated at the level of protein and mRNA expression in the LDIR exposed mono-cultured EBV-B cells and upregulated MSCs co-cultured EBV-B cells, suggesting a putative novel miRNA regulatory mechanism in the genetic control of the LDIR-induced stress response. These findings illustrate that LDIR exposure and the cell’s microenvironment can affect specific gene expression, resulting in altered protein expression.

Free Research Field

臨床検査医学