Identification of new biomarkers and therapeutic targets for lung cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 24501341
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: The University of Tokyo (2014); Shiga University of Medical Science (2012-2013)
• Principal Investigator: ATSUSHI TAKANO 東京大学, 医科学研究所, 特任講師 (50582607)
• Co-Investigator(Kenkyū-buntansha): DAIGO Yataro 滋賀医科大学, 医学部, 教授 (30325029)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオマーカー

Outline of Final Research Achievements

We identified a secreted proteins, LASEP1 and LASEP3 (LASEPs) as a candidate. Immunohistochemical staining showed that high level of LASEPs expression was associated with poor prognosis for NSCLC patients. Serum LASEPs levels were higher in NSCLC patients than in healthy volunteers. Furthermore, reduction of LASEPs by siRNA suppressed lung cancer cell proliferation and induced apoptosis. In addition, subsequent microarray analysis of these cancer cells identified several candidate downstream genes of LASEPs that relate to cell growth signals.
Next generation sequencer analysis detected tumor-specific mutation in lung cancer tissue. In addition, we detected mutation in mRNA from serum exosome or free DNA from serum by digital PCR. Digital PCR analysis showed that activating EGFR mutation in serum exosome was detected 4 cases out of 10.

Free Research Field

腫瘍内科学