2014 Fiscal Year Final Research Report
Developments of A New Antibody Library and Antibody Chips
| Project/Area Number |
24501343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | EGFレセプター / 抗体 / ライブラリー |
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| Outline of Final Research Achievements |
(1)We have analyzed effects on tumor cells of anti-EGF receptor antibodies recognized unique epitopes, which were obtained form our original antibody library. The antibodies induced apoptosis to EGFR-overproduced tumor cells with wild type and several tumorigenic K-RAS mutations. The antibodies inhibited EGF-induced EGFR phosphorylation and Akt phosphorylation.
(2)For we have constructed an Fab antibody-displying phage library with 10^18 repertory which are based on new concept for library-screening system.
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| Free Research Field |
細胞生物学
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