2014 Fiscal Year Final Research Report
Telomere repeat-containing RNA (TERRA), a possible molecular marker of leukemia
| Project/Area Number |
24501344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
UMEZU Tomohiro 東京医科大学, 医学部, 講師 (40385547)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | テロメアRNA / 5-アザシチジン / 骨髄異形成症候群 |
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| Outline of Final Research Achievements |
Telomere repeat-containing RNAs (TERRAs), which are RNAs that originate from telomeric DNA transcription, can associate with the telomeric chromatin, where they are proposed to function as negative regulators of telomere length. In the current study, we established a RNA-FISH method for TERRA, and investigated the possible association between TERRA and 5-azacytidine (AZA), which is currently approved for the treatment of myeloid neoplasms. We found a constitutive up-regulation of TERRA in 2 AZA-resistant cell lines as well as bone marrow cells obtained from 3 AZA-resistant patients. These finding indicate that the RNA-FISH method for TERRA is useful not only to better understand molecular mechanism of AZA resistant but also to estimate AZA-resistant patients in clinical practice.
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| Free Research Field |
分子腫瘍学
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