Trial for identification of targeting molecules in malignant pleural mesothelioma by proteomics

2014 Fiscal Year Final Research Report

• Project/Area Number: 24501352
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical oncology
• Research Institution: Yamaguchi University
• Principal Investigator: KURAMITSU Yasuhiro 山口大学, 医学(系)研究科(研究院), 准教授 (50281811)
• Co-Investigator(Kenkyū-buntansha): UEDA Kazuhiro 山口大学, 医学部附属病院, 講師 (90420520)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオマーカー / 悪性胸膜中皮腫 / 分子標的 / プロテオミクス

Outline of Final Research Achievements

Proteomic differential display between malignant pleural mesothelioma cells and normal pleural cell identified HSP70、GRP78、PDX2、Protein disulfide isomerase A3、annexin A3 and 14kDa phosphohistidine phosphatase as up-regulated proteins in malignant pleural mesothelioma cells compared to normal pleural cell. Furthermore, by means of western blot analysis it was shown that in malignant pleural mesothelioma cells DDX39 was up-regulated compared to normal pleural cell. However, after knocking down these candidates the proliferation and moving were not down-regulated. After this we would like to investigate the function of these identified proteins in the tumorigenesis and pathogenesis of malignant pleural mesothelioma.

Free Research Field

腫瘍学