Development of novel cancer therapy by functional up-regulation of dystroglycan using glycosyltransferase LARGE

2014 Fiscal Year Final Research Report

• Project/Area Number: 24501357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical oncology
• Research Institution: Teikyo University
• Principal Investigator: SHIMIZU Teruo 帝京大学, 医療技術学部, 教授 (00107666)
• Co-Investigator(Renkei-kenkyūsha): MATSUMURA Kiichiro 帝京大学, 医学部, 教授 (50260922) ; SAITO Fumiaki 帝京大学, 医学部, 准教授 (40286993)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 癌 / 糖鎖 / ジストログリカン / ラミニン / LARGE

Outline of Final Research Achievements

It has been demonstrated that laminin binding activity of α-dystroglycan (α-DG) is reduced in many cancer cells by the defective glycosylation of α-DG and glycosyltransferase LARGE hyperglycosylates and increases its laminin binding activity. In this study, we investigated possible therapeutic strategy for cancer using LARGE. We demonstrated that, in addition to the increment of laminin binding activity, LARGE suppresses proliferation and invasion of cancer cells by modulating gene expression pattern involved in them. However, overexpression of LARGE inhibited differentiation of non-cancer cells, such as myoblasts. Therefore, further experiments are necessary to apply LARGE for the cancer therapy.

Free Research Field

神経内科学