The chaperone and its binding protein, annexin II, which have a distinct role in the extracellular space and in the nucleus, depending on the differences in radiation doses

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Chiba University
• Principal Investigator: KITA Kazuko 千葉大学, 医学(系)研究科(研究院), 講師 (80302545)
• Co-Investigator(Kenkyū-buntansha): SUGAYA Shigeru 千葉大学, 大学院医学研究院, 助教 (90334177)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Takeshi 千葉大学, 大学院医学研究院, 助教 (80595976)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: annexin II / シャペロン / 放射線 / 酸化ストレス / シグナル伝達 / DNA修復

Outline of Final Research Achievements

Annexin II, the chaperone HSP27-binding protein in the cytoplasm, is known to be localized in the extracellular space or in the nucleus of human cells. The mechanisms of the localization and its functions in the extracellular space or in the nucleus are not yet fully understood.
The current study is the first report to suggest that the reactive oxygen species (ROS)-activated signal pathway via p38 MAPK was involved in the extracellular release of annexin II, and this pathway was stimulated by low-dose X-ray irradiation. Furthermore, we found that pancreatic cancer-derived cells released annexin II more abundantly than other cancerous or noncancerous cells did, and our finding suggested the possibility that extracellular annexin II confers resistance to anticancer drugs and X-ray irradiation via PI3K and MEK signal pathways. We also suggested a novel potential role of annexin II in the nuclei that is associated with repair activity of damaged-DNA

Free Research Field

放射線生物学