A paradigm shift by two Ku80: New model of the radiation-induced DNA damage response mechanism in human cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510077
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: KOIKE MANABU 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 主任研究員 (70280740)
• Co-Investigator(Renkei-kenkyūsha): KOIKE Aki 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 技術員 (50415410)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Ku80

Outline of Final Research Achievements

Ku (the heterodimer of Ku70 and Ku80) plays a pivotal role in the DNA double-strand break (DSB) repair pathway (non-homologous end-joining (NHEJ). It has reported that two isoforms of Ku80 encoded by the same genes are expressed and function in primate cells, but not in rodent cells. In this study, our data suggested the possibility that nuclear localization signal of one isoform is localized not only in the C-terminal region, but also in the N-terminal region. In addition, we generated a transgenic mouse that were introduced GFP-KARP-1(1-88). These information and the mouse generated might be useful for study of KARP-1(1-88) functions and the DSB repair pathway.

Free Research Field

放射線生物学