2014 Fiscal Year Final Research Report
Structural analysis of the binding mechanism of the Ku protein to DNA that contains overhangs in its end
| Project/Area Number |
24510078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Manabu 独立行政法人放射線医学総合研究所, 放射線障害研究グループ, 主任研究員 (70280740)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | DNA損傷修復 / DNA二本鎖切断 / Ku / 分子シミュレーション / ライブセルイメージング |
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| Outline of Final Research Achievements |
The binding mechanism of the repair enzyme, Ku, to DNA that has overhangs in its 5’- or 3’- end was examined by both computational and experimental techniques. Comparing with the interaction between Ku and DNA with blunt ends, no significant differences were observed in the binding energy between Ku and DNA, but an amino acid residue of Ku that would interact with the terminal region with single-stranded DNA was identified. In addition, we established the system to examine the dynamics of Ku70 and Ku80, subunits of Ku protein, in the nucleus of cultured cells using the live cell imaging method, and succeeded in observing the accumulation of Ku70 or Ku80 at DNA damage sites produced by laser-microirradiation.
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| Free Research Field |
放射線生物学，計算化学
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