Development of 19F-NMR-Based Multiplex Focused Gene Diagnostic Systems

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: University of Tsukuba
• Principal Investigator: OISHI Motoi 筑波大学, 数理物質系, 講師 (90419242)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 19Ｆ－ＮＭＲ / ＤＮＡ / 磁性粒子 / 金ナノ粒子

Outline of Final Research Achievements

An ultrasensitive colorimetric assay for both DNA and RNA has been developed utilizing enzyme-free click chemical ligation chain reaction (CCLCR) on dispersed gold nanoparticles and magnetic beads. The CCLCR assay provides ultrasensitive detection (50 zM = 10-21 M: several copies) of target DNA that is comparable to PCR-based approaches. Note that target RNA could also be detected with similar sensitivity without the need for reverse transcription to the corresponding cDNA. Thus, this method might be a useful tool for development of 19F-NMR–based multiplex focused gene diagnostic systems.

Free Research Field

ナノバイオサイエンス